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Endoglycosidase S from Streptococcus pyogenes, Recombinant

Cat No.
NATE-1603
Description
Endoglycosidase S is specific for N-glycans attached on the Fc domain of IgGs. The N-glycan is cleaved after the first Glc-Nac. Reaction is mild and runs fast under physiological conditions without the need for co-factors or detergent. The enzyme only hydrolyze glycans from the Fc-domain of IgGs even if other glycosylated proteins are present in solution.
Abbr
Endo S, Recombinant (Streptocoocus pyogenes)
Source
E.coli
Species
Streptocoocus pyogenes
Appearance
White to light yellow powder
Form
Lyophilized powder with no preservatives added
Molecular Weight
110 kDa
Purity
> 95% homogeneity as determined by SDS-PAGE analysis.
Unit Definition
One unit digests > 95% of 1μg IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30min.
Optimum pH
6.5-7.5
Stability
The enzyme is reconstituted by addition of water and after reconstitution it is stable for 1 month at +4-8°C. The enzyme can be aliquoted and stored at -20°C for at least 6 months. The product is shipped at ambient temperature but should be stored at -20°C upon arrival.
Storage
at -20°C
Buffer
Cleavage buffer at pH 7.4 and at 37°C yields optimal enzyme activity. Many buffers at pH between 6-8 can be used but reaction conditions should be tested on a small amount before employing the enzyme at full scale.
Synonyms
Endoglycosidase S; IgGZERO
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