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Enzyme Activity Measurement for D-Arabinitol 4-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes offers reliable activity measurement for D-arabinitol 4-dehydrogenase. The quality of our results is assured with the most advanced spectrophotometric instruments. We surpass our competitors with a quick turnover of the activity assays in response to the request of either a typical activity determination or a customized task.

D-arabinitol 4-dehydrogenase (EC1.1.1.11) is a group of oxidoreductase enzymes that catalyze D-arabinitol and convert it to D-xylulose via reduction of the cofactor NAD (Nicotinamide Adenine Dinucleotide). The systematic name of this enzyme class is D-arabinitol:NAD+ 4-oxidoreductase. It is also named as dalD, D-arabitol dehydrogenase, and arabitol dehydrogenase. This enzyme is a member of the oxidoreductases family, more specifically, the oxidoreductases that catalyze the CH-OH group of the substrate and use NAD+ or NADP+ as the cofactor. It plays an important role in the biological pathways including pentose and glucuronate interconversions, sugar alcohol degradation, fructose and mannose metabolism, and other metabolic pathways. The enzyme thus has a significant function in many fields such as the chemical and pharmaceutical industries.

D-arabinitol 4-dehydrogenase was also reported to use other alcohols than D-arabinitol as the substrate, including D-arabitol, D-glucitol, D-mannitol, D-sorbitol, galactitol, and even ethanol. Because of the substrate promiscuity, activity assays of D-arabinitol dehydrogenase using spectrophotometry have not been well established in an industrial scale, although activity measurement using other methods were reported before. The spectrophotometric assay is considered to be the reliable and cost-effective method for activity quantification. Here, Creative Enzymes offers the most advanced spectrophotometric assays to measure the enzymatic activity accurately, which is backed by a skillful team of scientists. Creative Enzymes is your trustworthy partner during development of products containing D-arabinitol 4-dehydrogenase.

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