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Enzyme Activity Measurement for Estradiol 17Beta-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes offers the precise measurement of enzyme activity by spectrophotometric analysis, especially the assay for estradiol 17beta-dehydrogenase. Although the test method is not available to most companies, we, as the expertise of enzymology, can accurately measure and characterize estradiol 17beta-dehydrogenase.

Estradiol 17beta-dehydrogenase (EC1.1.1.62) is an enzyme that catalyzes the oxidation of 17beta-estradiol to produce estrone, using NAD+/NADP+ as the redox cofactor. The enzyme exists in human and many other mammals, including Bos taurus, Gallus gallus, Mus musculus, and Sus scrofa. This enzyme is a member of the general oxidoreductase family, which catalyze the CH-OH group of the substrate and use NAD+ or NADP+ as the cofactor. The systematic name of this enzyme class is estradiol-17beta:NAD(P)+ 17-oxidoreductase. And other names in common use include:

Estradiol 17beta-dehydrogenase is involved in the pathway of estradiol biosynthesis I (via estrone), which is part of the superpathway of steroid hormone biosynthesis. Estrogens are steroid hormones which are synthesized in the ovaries, breasts, adipose, and other tissues. They are critical to the sexual development , as well as the function of sexual organs. Estrogens can also act as inducers of tumor growth in estrogen dependent cancerous tissues. They thus become the target for many anti-cancer therapies. 17β-estradiol is the main estrogen in human, which has a high estrogenic effect. The increased estrogen levels during pregnancy may be related to higher risks of breast cancer. Consequently, enzymes in this pathway, especially estradiol 17beta-dehydrogenase, is the target of existing or potential cancer therapies. Despite of the promising future of estradiol 17beta-dehydrogenase in biological and pharmaceutical industries, the activity assay of this enzyme has not been well developed because of the availability of substrates and lack of knowledge of the enzyme. Meanwhile, Creative Enzymes independently developed reliable activity assays for this enzyme, based on deep understanding of its structure and properties. The catalytic activity of estradiol 17beta-dehydrogenase can be measured by following either the reduction of NAD+ at 260nm or the oxidation of NADH at 340nm, using the spectrophotometric analysis. Overall, Creative Enzymes is your best choice for any research and development activities on estradiol 17beta-dehydrogenase.

Enzyme Activity Measurement for Estradiol 17Beta-Dehydrogenase Using Spectrophotometric Assays Figure: The crystal structure of estradiol 17-beta-dehydrogenase 1 with A-diol. PDB: 3KLP

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