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Enzyme Activity Measurement for Inositol 2-Dehydrogenase Using Spectrophotometric Assays

Based on a strong team of experienced enzymologists, Creative Enzymes has become a leader in designing activity assays to satisfy various needs of enzyme detection and characterization. The reliability of our results is assured by the spectrophotometric technology that is the vanguard of times. We impressed our customers by the highly accurate and reproducible measurement, especially for the activity of inositol 2-dehydrogenase.

Inositol 2-dehydrogenase (EC 1.1.1.18) is a member of the family of oxidoreductases, specifically those acting on the CH-OH group as the electron donor with NAD+ (nicotinamide adenine dinucleotide) or NADP+ (nicotinamide adenine dinucleotide phosphate) as the electron acceptor. It catalyzes the NAD-dependent oxidation reaction that converts myo-inositol, a carbocyclic polyol, to 2,4,6/3,5-pentahydroxycyclohexanone. The enzyme is involved in inositol metabolism and inositol phosphate metabolism. It is widely known that myo-inositol, the most prominent natural form of inositol, works as the structural basis of inositol phosphates, which plays a skeletal role related to various secondary messengers in eukaryotic cells, as well as cell growth, apoptosis, cell migration, endocytosis, and cell differentiation. It is also reported that this reaction is involved in some other metabolic pathways, such as streptomycin biosynthesis, antibiotics biosynthesis, and microbial metabolism in diverse environments. The systematic name of this enzyme class is myo-inositol:NAD+ 2-oxidoreductase. Other names of this enzyme commonly used include:

The chemical reaction catalyzed by inositol 2-dehydrogenase requires the presence of NAD+. Therefore, spectrophotometric assays can be used to measure the enzymatic activity of inositol 2-dehydrogenase continuously by monitoring increases of absorbance at 340 nm. Although activity assays are commercially available, the current assays are based on the natural substrate at a certain concentration. However, the growing demands in life sciences and biotechnology often require a more flexible test method against various unnatural substrates. Creative Enzymes has a strong background on custom test designs and is able to provide activity measurement of the pure enzyme or as a blend against a wide range of substrates and substrate levels. We have established a unique test platform and is happy to offer spectrophotometric assays for inositol 2-dehydrogenase.

Enzyme Activity Measurement for Inositol 2-Dehydrogenase Using Spectrophotometric Assays Figure: The crystal structure of myo-inositol dehydrogenase from Lactobacillus casei, including molecules of inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase and bound cofactor NAD.



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