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Enzyme Activity Measurement for Manganese Peroxidase

Creative Enzymes is the leading company in the field of enzymatic assays, which is well known to provide the best customer satisfaction. With many years of experiences in testing enzymes and optimizing methods, Creative Enzymes promises to return your enzyme kinetic data in a precise and timely manner. Here we are proud to offer the most precise activity measurement for manganese peroxidase.

Manganese peroxidase (EC; MnP) is the most common lignin-modifying peroxidase produced by nearly all wood-colonizing basidiomycetes causing white-rot and kinds of soil-colonizing litter-decomposing fungi. Different forms of this glycosylated heme protein are secreted by ligninolytic fungi into their microenvironment and manganese peroxidase was first discovered in the fungus Phanerochaete chrysoporium in 1985. Manganese peroxidase from P. chrysoporium has been characterized extensively by a variety of biochemical and biophysical methods. In addition, the sequences of cDNA and genomic clones encoding several P. chrysosporium manganese peroxidase isozymes have been determined. Biophysical studies and DNA sequences illustrate that the heme environment and catalytic cycle of manganese peroxidase are similar to those of other heme peroxidases, such as the horseradish peroxidase. However, manganese peroxidase is unique in its ability to catalyze the one-electron oxidation of Mn2+ to Mn3+ in a multi-step reaction cycle:

MnP + H2O2 → MnP compound I + H2O (1)

MnP compound I + Mn2+ → MnP compound II + Mn3+ (2)

MnP compound II + Mn2+ → MnP + Mn3+ + H2O (3)

Chelated Mn3+, in turn, acts as a diffusible redox-mediator that attacks phenolic lignin structures resulting in the formation of unstable free radicals that tend to disintegrate spontaneously.

The remarkable degradative potential of manganese peroxidase makes this enzyme attractive for biotechnological applications, e.g. in pulping and bleaching of cellulose, in removing of parlous wastes or in certain organic syntheses. Likewise, manganese peroxidase has various prospective applications in bioremediation, analytic biochemistry, and many industries that include food, cosmetics, textile and dye.

The crystal structure of manganese peroxidase from P. chrysosporium. Figure: The crystal structure of manganese peroxidase from P. chrysosporium.

In order to have a better and thorough understanding of the catalytic cycle of manganese peroxidase, determination of the enzyme activity is critically important. Nevertheless, the activity assay for this enzyme was reported to be complex probably because of the multiple steps involved in catalysis. Creative Enzymes is one of the few companies which have enough ability to offer the enzymatic assays for manganese peroxidase. It is worth mentioning that Creative Enzymes can handle all complicated situations in your research, and provides you with customized services and accurate test results of enzyme assays.

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