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Enzyme Activity Measurement of 3-Hydroxyacyl-CoA Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes provides enzymatic activity measurement for a variety of enzymes. We have been a leading company in providing accurate and reproducible spectrophotometric quantification services. A high standard of operation and execution is maintained through all steps during the measurement. We have established a premier benchmark in activity assays for oxidoreductases, including 3-hydroxyacyl-CoA dehydrogenase.

3-Hydroxyacyl-CoA dehydrogenase (EC is the penultimate enzyme in the β-
oxidation spiral, catalyzing the oxidation of the hydroxyl group of 3-hydroxyacyl-CoA to a keto group, concomitant with the reduction of NAD+ to NADH. This enzyme belongs to the family of oxidoreductases. Alternative names of the enzymes are as follows:

Enzyme Activity Measurement of 3-Hydroxyacyl-CoA Dehydrogenase Using Spectrophotometric Assays Figure: The structure of 3-hydroxyacyl-CoA dehydrogenase complexed with acetoacetyl-CoA and NAD+.

The structure of human 3-hydroxyacyl-CoA dehydrogenase complexed with NAD+ shows the subunits of the dimeric enzyme are composed of two domains: The NAD+-binding domain and the C-terminal domain, which is primarily α-helical and mediates subunit dimerization. The studies on the structure facilitated understanding of the catalytic mechanism of this enzyme in fatty acid metabolic processes, which is the most important in human metabolic pathway. As the enzyme catalyzes the third step of beta oxidation, 3-hydroxyacyl-CoA dehydrogenase collaborates with other enzymes to from two-carbon-less carbon chains. This progress is accompanied by the generation of energy. Beta oxidation is a circle oxidation pathway and ends up with the generation of acetyl-CoA, which eventually enters the citric acid cycle. Therefore, research on this enzyme contributes to realization of catabolism in most organisms and benefits the pathway design where the metabolism network often needs to be elaborated in synthetic biology. Increasing studies on this enzyme needs highly professional enzyme activity assays to support the subsequent research. To serve customers, Creative Enzymes provides reliable tests on 3-hydroxyacyl-CoA dehydrogenase activity measurement, using spectrophotometric assays.

Creative Enzymes is specialized in enzyme activity measurement for several years. We have the cutting-edge equipment and extensive experiences which allow us to maintain a leading position in this field. Creative Enzymes promises high reproducibility and reliable results for every testing request.

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