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Enzyme Activity Measurement of D-Galactose 1-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes is one of the few companies that are capable ofproviding high-quality enzyme activity assays. The advanced equipment and specialized working team support us to accomplish proper activity measurement with rapid turnover and high accuracy. We have years of experiences in oxidoreductase testing, including D-galactose 1-dehydrogenase.

D-galactose 1-dehydrogenase catalyzes the transformation from D-galactose to D-galactono-1,4-lactone, accompanied with the generation of NADH. This enzyme belongs to the family of oxidoreductases and it has been found in many bacteria, such as Azotobacter vinelandii. Other names for common use are:

This enzyme participates in D-galactose degradation II pathway. In many microorganisms, cells can utilize D-galactose for growth via two alternative pathways, one is the Leloir pathway, another is De Ley-Doudoroff pathway. The De Ley-Doudoroff pathway was first described in Pelomonas saccharophila, and it has been found in a few bacterial species. This pathway oxidizes D-galactose to a lactone, followed by enzymatic hydrolysis of the lactone to galactonate. Galatonate is eventually converted to D-glyceraldehyde-3-phosphate and pyruvate, both the major metabolites in the glycolysis pathway. The key enzyme in the De Ley-Doudoroff pathway is the initial enzyme, D-galactose dehydrogenase, which is the central player of the first step in De Ley-Doudoroff pathway. This enzyme also takes parts in other metabolic pathways, such as galactose metabolism. Due to the multiple roles it has in D-galactose utilization, D-galactose 1-dehydrogenase has become a potential target of bacteriological studies on the growing mechanism using D-galactose. Proper analysis methods for activity quantification for D-galactose 1-dehydrogenase is necessary to support the research related to this enzyme and the De Ley-Doudoroff pathway. We have demonstrated that a most suitable testing method is the spectrophotometric assay, which can be performed efficiently by detecting the absorption of NADH at 340nm.

Enzyme Activity Measurement of D-Galactose 1-Dehydrogenase Using Spectrophotometric Assays Figure: The crystal structure of D-galactose-1-dehydrogenase protein from Rhizobium etli.

Creative Enzymes provides the high-quality services for enzymatic activity measurements, and reliability of the results is beyond the typical level of the industry. Our precise testing results are rely on the advanced testing instrument and professional technical team. Creative Enzymes is your best choice for enzyme activity measurement.

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