Services

Professional and Cost-Saving Solutions

Enzyme Activity Measurement of D-Lactate Dehydrogenase Using Spectrophotometric Assays



Creative enzyme is committed to providing customers with high-quality enzyme activity assay services. We are proud of our professional technology, excellent team, and fast and efficient detection of enzyme activity, including D-lactate dehydrogenase.

D-lactate dehydrogenases and L-lactate dehydrogenases (D-LDH and L-LDH) reduce pyruvate to D- and L-lactate, respectively, with oxidization of NADH into NAD+, and act as the last step of the glycolytic pathway under anaerobic conditions. D-lactate dehydrogenase is also known as:

D-LDHs belongs to a large D-2-hydroxyacid dehydrogenase (D-HydDH) superfamily, which comprises various types of D-isomer dehydrogenases, such as the D-glycerate, D-3-phosphoglycerate, D-2-hydroxyglutarate and D-2-hydroxyisocaproate dehydrogenases. Although these enzymes bear highly divergent primary sequences, their tertiary structures are quite similar to one another. The Lactobacillus D-LDHs consistently have a dimeric structure comprising identical subunits, and exhibit hyperbolic shaped saturation curves for substrate pyruvate. And in E. coli cells, D-LDH gives a homotetrameric structure. Bacterial allosteric D-LDHs are commonly activated by FBP (fructose 1,6-bisphosphate) and the inhibition occurres when the oxmate presents in the solvent. D-lactate dehydrogenases activity was determined by measuring the variation of the absorbance at 340 nm derived from NADH. Creative Enzymes offers rapid and accurate assay methods to detect not only purified D-lactate dehydrogenases, but also samples in serum, plasma, cell lysates, culture and fermentation media.

Enzyme Activity Measurement of D-Lactate Dehydrogenase Using Spectrophotometric Assays
Figure: The crystal structure of D-lactate dehydrogenase from Escherichia coli.

Reference: Furukawa et al. AMB Express 2014, 4:76

Creative Enzymes is exceptionally skilled at providing assay services and proficient in activity measurement using spectrophotometry, or UV/vis spectroscopy in this case. Professional techniques and extensive experiences support us stand at the forefront of the enzyme activity assay industry. In the future, Creative Enzymes will continue to be the reliable partner in every aspect of your need with activity assays.



Inquiry
Related Products
CatalogNATE-1654
EC No.EC 1.1.1.28
CAS No.
SourceE. coli
CatalogEXWM-0187
EC No.EC 1.1.1.28
CAS No.9028-36-8
Source
CatalogNATE-1104
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceLeuconostoc mes...
CatalogNATE-1042
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceE. coli
CatalogNATE-0196
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceLeuconostoc mes...
CatalogNATE-0197
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceStaphylococcus ...
CatalogNATE-0195
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceLactobacillus l...
CatalogDIA-207
EC No.EC 1.1.1.28
CAS No.9028-36-8
SourceMicroorganism
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for private therapeutic use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2019 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: