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Enzyme Activity Measurement of Malate Dehydrogenase (Decarboxylating) Using Spectrophotometric Assays

Creative Enzymes is specialized in providing enzyme activity measurement services to assist scientists’ research in a rapid and accurate way. The professional processes and high-quality services of Creative Enzymes support researchers and innovators in various activity assays for oxidoreductases, including malate dehydrogenase (decarboxylating).

Malate dehydrogenase (decarboxylating) activity was first isolated from pigeon liver and has since been found in most living organisms, from bacteria to humans. Malate dehydrogenase (decarboxylating) (EC catalyzes the reversible oxidative decarboxylation of L-malate to pyruvate and CO2 with the concomitant reduction of the cofactor NAD+ or NADP+. The reaction generally proceeds in two steps – dehydrogenation (oxidation) of malate to produce the oxaloacetate intermediate, and decarboxylation to produce pyruvate – but, interestingly, the enzyme does not decarboxylate oxaloacetate that is added exogenously. The enzyme requires divalent cations (Mg2+, Mn2+, or others) in the catalysis. Other common names of this enzyme include:

The amino acid sequences of malic enzymes are highly conversed across all the organisms. The wide distribution of the enzymatic activity in nature and the high degree of sequence conservation are consistent with the important biological functions of these enzymes, such as photosynthesis in C4 plants and even some C3 plants; and biosynthesis of fatty acids and steroids in liver and adipose tissues in animals. Due to the important biological position of malic enzymes, the studies on this enzyme have grown greatly interested. Having taking into account that the increasing needs of activity determination for malate dehydrogenase (oxaloacetate-decarboxylating), Creative Enzymes provides accurate and rapid activity testing services to business and institutions of all sizes and specialties. Our leading-edge enzymology assays employ the modern spectrophotometric assay to keep high reliability of the test results.

Enzyme Activity Measurement of Malate Dehydrogenase (Decarboxylating) Using Spectrophotometric Assays Figure: The crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme in a pentary complex with natural substrate malate, cofactor NADH, Mn2+, and allosteric activator fumarate.

Reference: Xiao tao et al. Structure. 2003 11(9):1141-50

Creative Enzymes is one of the few companies providing activity assay services focusing on malate dehydrogenase (decarboxylating). Our unparalleled high-quality tests guarantee reliable and reproducible results. Creative Enzymes is your best choice for activity measurement.

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