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Enzyme Activity Measurement of N-Acylmannosamine 1-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes devotes complete efforts to enzyme assay services. We lead the enzymology service industry with the top equipment and professional research team. We accumulated extensive experiences through repeatedly testing enzyme activities for oxidoreductases. Therefore, we are able to offer rapid and reliable assay services for enzymatic activity determination, including N-acylmannosamine 1-dehydrogenase.

N-acylmannosamine 1-dehydrogenase belongs to the SDR (short-chain dehydrogenase/reductase) superfamily, and it catalyzes a rare NAD+-dependent oxidation reaction of ManNAc (N-acetyl-Dmannosamine) and turns it into N-acetylmannosamino-lactone, which spontaneously hydrolyzes into N-acetylmannosaminic acid. This enzyme hasn’t been found in other organisms than Flavobacterium sp, and it is so far the only enzyme that catalyzes the reaction. The enzyme is also called by other terms:

N-acylmannosamine 1-dehydrogenase is involved in sialic acid catabolism in bacteria. The most common form of sialic acid is Neu5Ac (N-acetylneuraminic acid), which is considered as a biomarker for some tumoral and inflammatory states, rendering its quantification in serum important. In microgranisms, Neu5Ac splits to pyruvate and ManNAc. Usually, biological processes converse this intermediate into fructose 1,6-bisphosphate through a series of enzymes catalyze. However, there is a unique pathway exists in a soil bacterium Flavobacterium sp. 141-8, which is correlated to its ability to grow on ManNAc as the sole carbon source. The unique enzyme is N-acyl-D-mannosamine dehydrogenase, which has not been found in any other microorganism. Little studies on this unique pathway has been done, and the consecutive reactions following N-acetylmannosaminic acid still remain unknown. Thus, scientists are keen to understand this enzyme and pathway. This enzyme is currently sold commercially as a part of the sialic acid assay kit for clinical testing. While many other potential applications awaits until the enzyme is better studied and characterized. Whether your interest lies in pathway studies or enzyme development from new sources, Creative Enzymes will support your research by providing accurate enzyme assay services.

Enzyme Activity Measurement of N-Acylmannosamine 1-Dehydrogenase Using Spectrophotometric Assays
Figure: The crystal structure of the N-acetyl-D-mannosamine dehydrogenase with NAD from Flavobacterium sp. 141-8.
Reference: Agust´ın SOLA-CARVAJAL et al. Biochem. J. 2014 462, 499–511

Creative Enzymes is one of the few companies providing assay services for N-acylmannosamine 1-dehydrogenase. We have the most advanced spectrophotometric instrument and top-performance team. The excellent services have been approved by many customers. In the future, Creative Enzymes will persist in the best test quality to support your research.

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SourceE. coli
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