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Enzyme Activity Measurement of (R, R)-Butanediol Dehydrogenase Using Spectrophotometric Assays

Creative enzymes is a pioneer in (R, R)-butanediol dehydrogenase activity testing. (R, R)-butanediol Dehydrogenase (EC1.1.1.4) exists in both prokaryotic organisms, such as Bacillus and Enterobacter, and eukaryotic organisms, for example, Saccharomyces cerevisiae. The enzyme catalyzes the oxidation-reduction reaction and employs NAD as the cofactor. The advanced spectrophotometric instrument, including UV/vis spectrometers and colorimeter, and extensive experiences of Creative Enzymes guarantee high precision and little variations in the test result.

Enzyme Activity Measurement of (R, R)-Butanediol Dehydrogenase Using Spectrophotometric Assays Figure 1: The chemical reaction catalyzed by (R, R)-butanediol dehydrogenase.

Enzyme Activity Measurement of (R, R)-Butanediol Dehydrogenase Using Spectrophotometric Assays Figure 2: Meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae.

(R, R)-butanediol Dehydrogenase (EC 1.1.1.4) is involved in biosynthesis and degradation of (R, R)-butanediol. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of the donor with NAD+ or NADP+ as the acceptor. The enzyme, in its native role, participates in butanoic acid metabolism.

Different compounds having dissimilar atomic and molecular interactions have characteristic absorption phenomena and absorption spectra, which forms the basis of the spectrophotometric assay. Both NAD+ and NADH have specific UV absorption, so that the catalytic activity of EC 1.1.1.4 can be measured by following either the reduction of NAD+ at 260 nm or the oxidation of NADH at 340 nm, using spectrophotometric analysis. Spectrophotometric analysis is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light, near-ultraviolet, and near-infrared. Although spectrophotometric analysis can be interfered by many other molecules in solvent, Creative Enzymes always gives more targeted approaches combing the reality. We have demonstrated excellent sensitivity and steadiness of the spectrophotometric assays for these oxidoreductases under largely varied conditions.

Creative Enzymes has advanced testing equipment, the wealth of experiences, and a strong research team to support your laboratory research. Our unparalleled ability in enzyme activity testing has been endorsed by many customers and become the first choice of more and more researchers. The oxidoreductase activity assay service delivers robust and accurate results and is the choice worth trusted.



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Related Products
CatalogEXWM-0320
EC No.EC 1.1.1.4
CAS No.37250-09-2
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