Services

Professional and Cost-Saving Solutions

Enzyme Activity Measurement of Uracil/thymine Dehydrogenase



Creative Enzymes is a service provider especially engaged in enzyme activity measurement. We have the most advanced equipment and professional testing protocols. With years of extensive experiences, we can totally satisfy your demands in enzymology research. We are able provide reliable and reproducible results for enzymes, especially oxidoreductase such as uracil/thymine dehydrogenase.

Uracil/thymine dehydrogenase is one of the oxidoreductases that catalyze the chemical reactions targeting uracil and thymine and producing barbiturate and 5-methylbarbiturate respectively. Other molecules participate in the reaction are H2O and electron acceptors, which finally form the reduced acceptors. For example, 2,6-dichlorophenolindophenol can serve as an electron acceptor in Enterobacter aerogenes. This enzyme is now found only in some of the bacteria. Other alternative names in common use include:

There are two pathways of pyrimidine metabolism, one is reductive pathway appears in mammals, plants, and microorganisms, while, another one is the oxidative pathway. Uracil/thymine dehydrogenase is involved in uracil degradation II oxidative pathway and the degradation of thymine in some microorganisms, which is an important part in pyrimidine metabolism. The oxidative pathway of pyrimidine degradation was first reported in 1952, in Mycobacterium, Corynebacterium, and Norcardia, isolated from soil. In this pathway, the catalyzed products of uracil/thymine dehydrogenase, namely barbiturate and 5-methylbarbiturate, are subsequently converted to urea and malonic acid by barbiturase. However, the precise process towards urea and malonic acid is unclear yet, which becomes a bottleneck for further studies of the oxidative pathway. The proper enzyme activity assay for uracil/thymine dehydrogenase will support the follow-up studies to better understand this pathway. Some reports have shown that this enzyme is unstable in the cell-free extract, with a complete loss of activity within 3 days, which makes ex vivo activity measurement very difficult. To solve this challenge, Creative Enzymes developed the capability to provide rapid assay approaches and accurate result before enzyme inactivation.

Creative Enzymes belongs to the first tier in the enzyme service suppliers in the world. Our competitive cost performance ratio is your best choice for activity testing. We can test various forms of enzyme extractions and provide correct results. Overall, Creative Enzymes is your trustworthy partner in further research and development.

Enzyme Activity Measurement of Uracil/thymine Dehydrogenase Figure: The reaction catalyzed by uracil/thymine dehydrogenase in the case of uracil substrate.



Inquiry
Related Products
CatalogEXWM-1104
EC No.EC 1.17.99.4
CAS No.9029-00-9
Source
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for private therapeutic use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2019 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: