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Enzyme Activity Measurement of D-Xylulose Reductase Using Spectrophotometric Assays

Creative Enzymes is specialized in providing enzyme activity measurement services, especially the detection of oxidoreductases. Based on the most experienced experts and the most advanced equipment, Creative Enzymes gives the accurate result and the fastest assay service.

D-xylulose reductase (EC is one of several enzymes that are responsible for assimilating xylose into eukaryotic metabolism, and it is useful for ethanol production through fermentation of xylose containing agricultural byproducts. D-xylulose reductase (EC is also known by other names:

D-xylulose reductase oxidizes xylitol to form xylulose, using NAD+ exclusively as the co-substrate. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme is related to the pentose and glucuronate interconversion pathway in broad species of organisms. For efficient xylose utilization at high flux rates in this pathway, co-substrates are recycled between the NAD+-specific D-xylulose reductase and the NADPH-preferring xylose reductase, another enzyme in the same pathway.

Enzyme Activity Measurement of D-Xylulose Reductase Using Spectrophotometric Assays Figure: The structure of NAD+-bound xylitol dehydrogenase from Gluconobacter oxydans.
Reference: Andreas H. Ehrensberger et al., Structure 2006,14, 567–575

The metabolic integration of xylose plays a key role in the bioconversion of lignocellulose-containing agricultural waste products to ethanol. D-xylulose reductase is an essential part of this pathway. Therefore, the enzyme is supposed to become more widely used in biowaste valorization, production fermentation, and animal feed. Proper measurement of the enzymatic activity thus is in an increasing need. The catalytic activity of EC can be measured by following either the reduction of NAD+ at 260 nm or the oxidation of NADH at 340 nm. Creative Enzymes is specialized in enzyme activity measurement and can help to determine D-xylulose reductase activity using spectrophotometric methods.

Based on the extensive experiences, Creative Enzymes has become the leader in the enzyme activity assay field. Our advanced technology can satisfy special manufacturing based on client’s needs. Creative Enzymes is your trustworthy partner for activity measurement and you will be pleased by the superior service experience here.

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