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Enzyme Activity Measurement of Phosphogluconate 2-Dehydrogenase Using Spectrophotometric Assays

Creative Enzymes is a leading company in the enzyme assay industry, which provides accurate and reliable testing results. Relying on the advanced equipment and knowledgeable specialists, Creative Enzymes tests far exceed the average quality. Our assay services covers many oxidoreductases, including phosphogluconate 2-dehydrogenase.

Phosphogluconate 2-dehydrogenase, the third enzyme of the pentose phosphate pathway, catalyzes the oxidative decarboxylation of 6-phosphogluconate (6PG), giving ribulose 5-phosphate as the product, along with the reduction of NADP+ to NADPH and the release of CO2. The enzyme catalyzes the reaction via three steps: (1) oxidation of the C3-hydroxyl group of 6PG to a C3-keto intermediate, (2) decarboxylation to give 1,2-enediol and (3) simultaneous tautomerization to Ru5P. Other names of this enzyme include:

This enzyme participates in the pentose phosphate pathway. This pathway is a metabolic pathway parallel to glycolysis. Phosphogluconate 2-dehydrogenase catalyzed reaction generates reducing equivalents, in the form of NADPH through oxidation, which then provides the reducing power for the synthesis reaction in cells, such as the synthesis of fatty acids. This pathway also produces ribose 5-phosphate (R5P) and erythrose 4-phosphate (E4P), which provide materials for the synthesis of many biological building blocks, such as nucleotides. This enzyme is also involved in glutathione metabolism. Glutathione is an important antioxidant, which is capable of preventing damages to important cellular components from reactive oxygen species, such as free radicals, peroxides, lipid peroxides, and heavy metals. Therefore, it can be seen that phosphogluconate 2-dehydrogenase plays a key role in metabolism and ensures vitality of organisms. Due to the important function in cells, an increasing number of researchers focus their studies on this enzyme. It is an urgent demand to set up proper assay methods for phosphogluconate 2-dehydrogenase. To this end, Creative Enzymes provides accurate and rapid assay methods for phosphogluconate 2-dehydrogenase, using the most advanced spectrophotometric instrument.

Enzyme Activity Measurement of Phosphogluconate 2-Dehydrogenase Using Spectrophotometric Assays Figure: The crystal structure of dimeric 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate.

Reference: Chen YY et al. J Struct Biol. 2010 169(1):25-35

Creative Enzymes is one of the best companies in enzyme activity assays. We can provide targeted protocols for specific demands, using the advantage of extensive experiences and professional techniques. In the future, Creative Enzymes will continue supporting your research with the-state-of-the-art service.

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