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Enzyme Activity Measurement of Arachidonate 15-Lipoxygenase



Creative Enzymes has a remarkable record of supplying efficient and reliable assay services. Our professional technical tests have been testified by tens of thousands of our customers. Based on the cutting-edge equipment and superb enzymologist team, the testing results for arachidonate 15-lipoxygenase are assured with precision.

Arachidonate 15-lipoxygenase is a member of lipoxygenases (LOX’s), which are nonheme iron dioxygenases. LOX’s exist in a great number of organisms, such as bacteria, plants, and mammals, and act on polyunsaturated fatty acids for their regioselective and stereospecific hydroperoxidation. Arachidonate 15-lipoxygenase catalyzes the conversion from arachidonate to (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate, with the incorporation of two atoms of oxygen. Subsequently, the product is rapidly reduced to the corresponding 15S-hydroxy compound. In addition to the arachidonic acid metabolism, this enzyme also participates in the linoleic metabolism pathway.

Widely distributed in animal and plant species, the enzyme displays different activities. In plants, it is involved in germination and senescence, while in humans it is related to a variety of diseases. An increasing number of studies suggest that this enzyme inhibits and resolves diverse inflammatory diseases, and the inhibitors, which are proved to be clinically useful, have been made to resist metabolic inactivation. As for asthma and other allergic diseases, this enzyme is the mainly target of future studies for potential treatments. As for cancer, the arachidonate 15-lipoxygenase level is low or absent compared to normal tissues. Furthermore, studies also showed that the enzyme level sharply declines as the cancer progresses. Studies in animal models suggested, but not proved, that this enzyme is a tumor suppressor due to mechanisms that are not identified yet. Given the clinical significance, more and more research on arachidonate 15-lipoxygenase has been initiated. The suitable and reliable activity testing methods could construct a bridge to satisfy the increasing amounts of urgent needs. Creative enzymes is proud to bring accurate assay services to facilitate such an effort.

 The structure of rabbit 15S-LOX1. Figure: The structure of rabbit 15S-LOX1.
Reference: Gillmor, S.A. et al. Nat.Struct.Biol. 1997 4: 1003-1009.

Creative Enzymes are well known for reliable quantification of enzyme activity. We stay at the top level of the enzyme industry because of the unique expertise and sophisticated test methods. Creative Enzymes will always stand by your side on the way to great achievements.



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