Enzyme Activity Measurement

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Creative Enzymes performs the most reliable measurement of enzyme activities for a variety of application purposes. Our unique technology platform delivers accurate, reproducible, and scalable results for all enzyme classes. With our vast library of enzymes, substrates, and cofactors, we ensure fast turnaround even for large-batch projects. Equipped with state-of-the-art analytical instruments and a rigorous data management system, we can handle even the most complex activity measurement requests, including cGMP and cGLP-compliant studies. Our knowledge, technology, and hardware together make Creative Enzymes a worldwide leader in enzyme activity measurement.

Why Enzyme Activity Measurement Matters

At Creative Enzymes, enzyme activity measurement isn't just a routine test—it's a precision service designed to reveal exactly how your enzymes perform in the real world. Every assay we run is engineered to overcome the challenges that can distort results, such as:

Low enzyme activity levels
Competition among substrates and cofactors
Weak reporter signal
Low detection sensitivity
Non-linear reaction rates
Interference from other components
Enzyme inhibition by other ingredients

Our team of enzymology specialists applies deep technical expertise to navigate these issues, ensuring your data is both reliable and application-ready.

Why Choose Creative Enzymes for Enzyme Activity Measurement

Comprehensive Enzyme Library

Enzymes, cofactors, substrates, and ligands for almost any assay.

Technology Flexibility

UV/Vis spectrophotometry, fluorometry, chemiluminescence, HPLC, LC-MS, microplate-based screening, etc.

Regulatory Compliance

Reliable and comprehensive cGMP & cGLP-capable testing.

Fast Turnaround

Optimized workflows for both small and large-scale projects.

Expert Consultation

In-depth data interpretation and assay optimization.

Comprehensive Coverage

Supports the comprehensive detection of various enzyme types.

Comprehensive Service Offerings

Service Workflow

Workflow of enzyme activity measurement

Service Delivery & Submission Guidelines

Item	Description
Enzyme Sample	Purified enzyme, crude extract, or formulated product Recommended quantity: 50 µg–5 mg (depending on assay sensitivity and scale) Storage/shipping conditions: Keep on ice or frozen, avoid repeated freeze-thaw cycles
Assay Information (if available)	Substrate(s), cofactors, buffer conditions Known inhibitors or interfering substances Preferred assay type (continuous vs. endpoint) or detection method
Project Objectives	Desired activity units (e.g., U/mL, k_cat, µmol/min) Application focus (research, drug discovery, industrial, diagnostic) Any regulatory requirements (cGMP, cGLP)
Additional Documentation (Optional but Recommended)	Enzyme sequence or protein ID for customized assay optimization Previous activity data or assay protocols Special handling instructions

Item	Description
Comprehensive Activity Data	Quantitative measurement of enzyme activity under your specified conditions Key kinetic parameters: K_m, V_max, k_cat Assessment of stability, pH optimum, temperature dependence, or inhibitor effects (if requested)
Tailored Assay Reports	Detailed methodology and experimental conditions Data visualization: graphs, charts, and tables Interpretation of results and recommendations for application or further optimization
Quality Assurance & Compliance	High reproducibility and precision Optional cGMP/cGLP-compliant documentation for regulatory or manufacturing use Confidential handling and secure data delivery
Expert Consultation	Guidance on assay selection, enzyme formulation, or troubleshooting Suggestions for future studies or scale-up activities

Our Service Scope

	Oxidoreductases Oxidoreductases Acting on Carbon Oxidoreductases Acting on Nitrogen and Sulfur Oxidoreductases Interacting with Inorganics
	Transferases Phosphorus Transferases Glycosyl, Hexosyl, and Pentosyl Transferases
	Hydrolases Hydrolases Acting on Ester Bonds Glycosylases
	Lyases
	Isomerases
	Ligases
	Kinases Screening & Profiling

Techniques & Methods

We provide quantitative assessments of catalytic efficiency under your specific conditions, using methods such as:

Continuous assays for real-time reaction monitoring
Endpoint assays for fixed-time measurement
Spectrophotometric, fluorometric, and luminescent assays for sensitive detection
Advanced analytical integrations like HPLC or LC–MS for complex matrices

Supported Applications

Our enzyme activity measurement service supports:

Drug Discovery: Inhibitor screening and mechanism studies
Industrial Enzymology: Biocatalyst optimization and process validation
Diagnostics: Clinical enzyme assays and quality control

With Creative Enzymes, you don't just get numbers—you get actionable insights that enhance reproducibility, accelerate development, and strengthen decision-making in both research and manufacturing.

Contact Our Team

Case Studies and Success Stories for Enzyme Activity Measurement

Case 1: Discovery of Two Novel Oxidases Using a High-Throughput Activity Screen

The discovery of new enzymes is both difficult and essential for biotechnology. Using a high-throughput screening approach, researchers tested 96 putative oxidases against 23 substrates and identified two novel enzymes. The first, N-acetyl-D-hexosamine oxidase (EC 1.1.3.29) from Ralstonia solanacearum, is a vanillyl alcohol oxidase-like flavoprotein active on N-acetylglucosamine and N-acetylgalactosamine; prior to this work, its activity was considered orphaned (known function without sequence). The second, a long-chain alcohol oxidase (EC 1.1.3.20) from an uncultured marine Euryarchaeota, is active on various fatty alcohols (preferentially 1-dodecanol) and shows no sequence similarity to known LCAOs, making it a novel representative of this enzyme class.

Figure 1. Characterization of HexNAcO from R. solanacearum. (B) Screen of HexNAcO activity in a range of buffers. (C) Screen of HexNAcO melting temperature in a range of buffers. (D) Activity of HexNAcO with GlcNAc upon 30-minute pre-incubation in a range of temperatures. (E) UV/Vis absorbance spectrum of purified HexNAcO. (Rembeza et al., 2022)

Case 2: Effects of Simulated Digestion on AABP-2B

This study examined the impact of simulated saliva–gastrointestinal digestion on Anemarrhena asphodeloides polysaccharide B (AABP-2B), focusing on its structural integrity, α-glucosidase inhibition, and influence on gut microbiota. Results showed that AABP-2B maintained its molecular weight and released no free monosaccharides, indicating resistance to degradation. Its inhibitory activity against α-glucosidase also remained intact, likely due to preserved structural features. In vitro fecal fermentation demonstrated that digested AABP-2B reshaped the gut microbiota, increasing beneficial genera such as Prevotella, Faecalibacterium, and Megasphaera, while suppressing pathogens. Additionally, short-chain fatty acid production increased significantly. These findings highlight AABP-2B's potential as a prebiotic or functional food for gut health.

Study of AABP-2B enzyme activity during digestion Figure 2. Changes in reducing sugars of AABP-2B during simulation of upper gastrointestinal digestion. (Chen et al., 2023)

FAQs About Enzyme Activity Measurement Services

Q: Can you measure enzymes with extremely low activity?

A: Yes. We use highly sensitive detection platforms—including fluorometric, luminescent, and HPLC-based methods—to quantify activity even at picomole levels. Our assay optimization ensures accurate results despite challenging detection limits.
Q: I don't have an existing assay for my enzyme. Can you develop one for me?

A: Absolutely. We specialize in custom assay development, from method selection and optimization to validation under your specific conditions. Our large library of substrates, cofactors, and ligands lets us quickly design and test suitable protocols.
Q: What kind of results will I receive?

A: You'll get a comprehensive report including raw data, calculated activity values, kinetic parameters (K_m, V_max, k_cat), experimental conditions, and professional interpretation. We also provide recommendations for further optimization or application-specific adjustments.
Q: Can you handle large-batch or high-throughput enzyme activity testing?

A: Yes. We are equipped for both small-scale research projects and industrial-scale QC testing, with the ability to process large batches efficiently without compromising precision.
Q: What information do you need from me to get started?

A: We require your enzyme sample, relevant assay conditions (if available), project objectives, and any special handling instructions. The more details you provide, the more quickly and precisely we can deliver results.
Q: Do you test enzymes for different industries?

A: Yes. Our enzyme activity measurement services cover pharmaceutical R&D, diagnostics, industrial biocatalysis, food & nutrition, personal care, and academic research. We tailor every assay to your industry's needs.

Whether for research, product development, or quality control, Creative Enzymes delivers precision, speed, and confidence in every measurement. Contact us today to discuss your enzyme activity measurement project.

References:

Chen J, Lan M, Zhang X, et al. Effects of simulated in vitro digestion on the structural characteristics, inhibitory activity on α-glucosidase, and fermentation behaviours of a polysaccharide from Anemarrhena asphodeloides bunge. Nutrients. 2023;15(8):1965. doi:10.3390/nu15081965
Rembeza E, Boverio A, Fraaije MW, Engqvist MKM. Discovery of two novel oxidases using a high‐throughput activity screen. ChemBioChem. 2022;23(2):e202100510. doi:10.1002/cbic.202100510

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