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Enzyme Activity Measurement of Arabinogalactan Endo-Beta-1,4-Galactanase

Among numerous enzyme service providers, Creative Enzymes is the most reliable for enzyme activity measurement. Equipped with the advanced equipment and comprehensive expertise, Creative Enzymes offers customized services for virtually any enzyme. Our services’ quality for hydrolases are approved by lots of scientists. We have confident to provide reliable activity measurement for arabinogalactan endo-beta-1,4-galactanase.

Endo-beta-1,4-galactanase (AGs, EC 3.2.1.89) is one of the enzymes that catalyze the degradation of type I arabinogalactans (AG). More specifically, this enzyme hydrolyzes alpha-1,4-linked galactan of type I AGs to galactose and galactose oligomers. Based on related studies, this enzyme is classified into glycoside hydrolase family 53.

Arabinogalactans are branched galactans to which arabinose residues are bound as side chains and can be grouped into two types based on the structures of their backbones. Arabinogalactans have many applications as additives. For example, they are mainly used as a replacement for Arabic gum, guar gum, and starch in food, cosmetic and pharmaceutical products. As a hydrolase, endo-beta-1,4-galactanase degrades AGs and contributes to utilization of the plant biomass. The activity measurement of this enzyme is carried out by measuring the release of reducing groups in the reaction mixture, using reliable spectrophotometric assays. At the research center of Creative Enzymes, the cutting-edge spectrophotometer assures rapid turnovers and accurate testing results. In addition, our talented experts established robust approaches using skillful techniques to fully satisfy various research demands.

The crystal structure of beta-1,4-galactanase from Aspergillus aculeatus at 293k Figure: The crystal structure of beta-1,4-galactanase from Aspergillus aculeatus at 293k.
Reference: Ryttersgaard, C. et al. Biochemistry 2002 41: 15135-15143.

Having testified by thousands of customers, Creative Enzymes is the best choice for enzyme activity determination. Relying on the extensive experience and the professional operations, we are able to solve all kinds of challenges in activity assays and deliver reliable and accurate results. In the future, Creative Enzymes will continue the superb services as your trustworthy partner.



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