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Enzyme Activity Measurement of Glucose-6-Phosphate Dehydrogenase (NADP+) Using Spectrophotometric Assays

Creative Enzymes is one of the top service providers who are engaged in enzymology research. Our services for enzymes activity measurement enjoy an outstanding reputation. The advanced equipment and up-to-date experiment methods guarantee rapid tests and accurate results. We are specialized in oxidoreductase activity assay, such as glucose-6-phosphate dehydrogenase (NADP+).

Glucose-6-phosphate dehydrogenase (NADP+) (EC 1.1.1.49) was first found by O. Warburg and F. Lipman in 1931, which further promoted the discovery of other metabolic pathways in addition to the glycolysis pathway. This enzyme is involved in the pentose phosphate pathway (PPP) (also named hexose monophosphate shunt, HMS), which is a vital mechanism for carbohydrate metabolism. EC 1.1.1.49 catalyzes the conversion from D-glucose 6-phosphate to 6-phospho-D-glucono-1,5-lactone, simultaneously with the conversion from NADP+ to NADPH. There are many common names such as:

The reaction catalyzed by glucose-6-phosphate dehydrogenase (NADP+) is the first step in PPP. This pathway is located in cell cytosol and possesses huge physiological significance. First of all, PPP is an important channel for cells to produce the reducing power, NADPH, which can then be used in the synthesis of fatty acids, amino acids, steroids, and other essential constructive materials for lives. Second, this pathway is the main source to generate different structures of sugar molecules and provide a condition for the mutual conversion among monosaccharides. Other important chemical molecules such as ATP, CoA, FAD, and ribose also come from this pathway. PPP widely exists in organisms, indicating that glucose-6-phosphate dehydrogenase (NADP+) is also distributed in many species from bacteria to humans. Cell growth and proliferation are all affected by glucose-6-phosphate dehydrogenase (NADP+). Noticing the clinical significance of this enzyme, more and more scientists focus their research interests on this enzyme. Proper enzymatic activity measurement is the key to in-depth studies on this enzyme. To this end, Creative Enzymes provides accurate testing approaches aiming at direct activity measurement even in a variety of enzymes extractions. The assay services are carried out using our most advanced spectrophotometric instrument.

Enzyme Activity Measurement of Glucose-6-Phosphate Dehydrogenase (NADP+) Using Spectrophotometric Assays Figure: The structure of Trypanosoma cruzi glucose-6-phosphate dehydrogenase (NADP+) in complex with D-glucose 6-phosphate and NADPH.
Reference: Mercaldi GF et al. FEBS Lett. 2016 590 (16): 2776-2786

Creative Enzymes is one of the few companies providing rapid and reliable assay services for glucose-6-phosphate dehydrogenase (NADP+). We are happy to assure you with accurate results in every test. In the future, Creative Enzymes will make every effort in perfecting enzyme activity assays to support your research.



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