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Enzyme Activity Measurement for Glucuronolactone Reductase Using Spectrophotometric Assays

Creative Enzymes provides spectrophotometric activity assays for glucuronolactone reductase. Due to its rare availability, the enzymatic activity of this enzyme has not been completely determined, and the assay method is yet to be better developed. Meanwhile, our professional enzymologists established assays exclusively for glucuronolactone reductase and offer reliable activity tests.

A glucuronolactone reductase (EC is an enzyme that catalyzes oxidation of L-gulono-1,4-lactone, using NADP as the redox cofactor, to produce D-glucurono-3,6-lactone. The systematic name of this enzyme class is L-gulono-1,4-lactone:NADP+ 1-oxidoreductase, and other names in common use include GRase, and gulonolactone dehydrogenase. The enzyme belongs to the family of oxidoreductases.  Glucuronolactone reductase also catalyzes the reversed reaction of D-glucurono-3,6-lactone with NADPH to produce L-gulono-1,4-lactone and NADP+.  It was first isolated from the liver of Rattus norvegicus (rat) as a microsomal enzyme, and it was showed as the key catalyst in the biological pathway of ascorbate and aldarate metabolism.

In addition to the natural substrate, the enzyme also catalyzes reduction of D-glucuronic acid, which is a building block of proteoglycans and glycoglycerolipids, such as heparin, chondroitin, keratan, and hyaluronic acid. Regulations of glucuronic acid contents through reduction by glucuronolactone reductase would largely change functions of blood, skin, and joints. On the other aspect, the product of reducing D-glucuronic acid is L-gulonic acid, which is found useful in chemical processing. Therefore, glucuronolactone reductase is gaining interests from innovators in cosmetic, health care, and chemical industries. To the contrary of its growing impact, the activity test of glucuronolactone reductase has not grown much towards commercialization, partially because the technical difficulties to provide a stable and pure reaction matrix. With the most prominent experts in the determination of enzyme activity, Creative Enzymes has been developing an ever-expanding portfolio of assays for enzymes. The spectrophotometric assay was showcased as a reliable method for activity quantification for glucuronolactone reductase.

Creative Enzymes is proud to be the first company who brought the most reliable activity assay for glucuronolactone reductase to an industrial standard. Whether your purpose is to treat an acute inflammation or to shine the healthy skin, using either pure glucuronolactone reductase or a mixture of an extract, Creative Enzymes is always your first choice of activity measurement. Your success will be supported by our top-quality services.

Enzyme Activity Measurement for Glucuronolactone Reductase Using Spectrophotometric Assays Figure: The reaction catalyzed by glucuronolactone reductase: L-gulono-1,4-lactone + NADP+ = D-lucurono-3,6-lactone + NADPH.

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