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Enzyme Activity Measurement of Oligosaccharide Reducing-End Xylanase



Creative Enzymes has been an active biotechnology company in the field of enzyme activity tests for many years and accumulated extensive experiences. The comprehensive expertise and the skilled operations guarantee the accuracy and the reproducibility of the test results. Our assay service received a large volume of commendations for its reliability. With the constant professional quality, Creative Enzymes now makes the assay service available for oligosaccharide reducing-end xylanase.

Glycoside hydrolases (GHs) are classified into more than a hundred families based on amino-acid sequence identity. Oligosaccharide reducing-end xylanase belongs to the GH8 family owing to its similarity to GH8 endo-xylanases. Oligosaccharide reducing-end xylanase (Rex, EC 3.2.1.156) is a unique exo-oligoxylanase that hydrolyzes xylo-oligosaccharide at their reducing ends to release xylose and leaving the new reducing end in the alpha configuration. It is specific for the beta anomers of xylooligosaccharides whose degree of polymerization is equal to or greater than 3. Up till now, Rex is the only complete exo-hydrolytic enzyme that releases monosaccharide from the reducing end.

Rex is originally isolated from the bacterium Bacillus and only the crystal structure from Bacillus halodurans has been identified. In this organism, the enzyme is considered to play a key role in the intracellular xylan metabolism by cleaving xylooligosaccharides. Because Rex is an inverting enzyme that produces the alpha-anomer at the reducing end, spontaneous mutarotation of the substrate is required for subsequent processing in vitro. It remains unclear whether there are any factors supporting the mutarotation of xylooligosaccharides in vivo or if there are any biological reasons for such an inefficient processing mechanism. Therefore, the studies are focused on the basic functional research. To this end, Creative Enzymes support with the excellent activity assay service. Enzyme activity quantification could be carried out by detecting the increase in xylose using performance ion exchange chromatography.

Enzyme Activity Measurement of Oligosaccharide Reducing-End Xylanase Figure: The crystal structure of reducing-end-xylose releasing exo-oligoxylanase.
Reference: Fushinobu, S. J.Biol.Chem. 2005 280: 17180-17186.

Creative Enzymes has a strong background on enzyme activity measurement and is the only company that provides assay services for oligosaccharide reducing-end xylanase. The equipment in our research center is of the latest model and operated through automation systems, which assures the shortest service time even for large batches of samples. You will enjoy superior service by choosing Creative Enzymes.



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